Coding

Part:BBa_K3807027:Design

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


GS gRNA expression cassette 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AtU6-26 ptomoter is an RNA Pol 3 promoter that is commonly used for RNA expression. Protospacer was designed to bind at the exon 11 of the glutamine synthtase gene and the gRNA bosy was taken from the original pEvolvR plasmid

Source

Designed in silico using sequence from Addgene plasmid #113077 and synthesized by Twist

References

Halperin et al, 2018